A multiplexed microflow LC–MS/MS-PRM assay for serologic quantification of IgG N- and HPX O- glycoforms in liver fibrosis

Aswini Panigrahi, Lihua Zhang, Julius Benicky, Miloslav Sanda, Jaeil Ahn, Radoslav Goldman

Sci Rep. 2023; 13: 606. Published online 2023 Jan 12. doi: 10.1038/s41598-023-27382-0

PMCID: PMC9837073

N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures

Julius Benicky, Miloslav Sanda, Zuzana Brnakova Kennedy, Radoslav Goldman

J Biol Chem. 2019 Nov 8; 294(45): 16816–16830. Published online 2019 Sep 26. doi: 10.1074/jbc.RA119.009989 PMCID: PMC6851326

Site specific analysis of changes in the glycosylation of proteins in liver cirrhosis using data independent workflow with soft fragmentation

Miloslav Sanda, Lihua Zhang, Nathan J. Edwards, Radoslav Goldman

Anal Bioanal Chem. Author manuscript; available in PMC 2018 Jan 1.

Published in final edited form as: Anal Bioanal Chem. 2017 Jan; 409(2): 619–627. Published online 2016 Nov 7. doi: 10.1007/s00216-016-0041-8 PMCID: PMC5370557

Increased sialylation of site specific O-glycoforms of hemopexin in liver disease

Miloslav Sanda, Julius Benicky, Jing Wu, Yiwen Wang, Kepher Makambi, Jaeil Ahn, Coleman I. Smith, Peng Zhao, Lihua Zhang, Radoslav Goldman

Clin Proteomics. 2016; 13: 24. Published online 2016 Sep 21. doi: 10.1186/s12014-016-9125-x

PMCID: PMC5034550

Analysis of site and structure specific core fucosylation in liver cirrhosis using exoglycosidase-assisted data-independent LC-MS/MS

Miloslav Sanda, Jaeil Ahn, Petr Kozlik, Radoslav Goldman

Sci Rep. 2021; 11: 23273. Published online 2021 Dec 2. doi: 10.1038/s41598-021-02838-3

PMCID: PMC8639754

Glycoforms of human prostate-specific membrane antigen (PSMA) in human cells and prostate tissue

Wei Yuan, Baoqin Liu, Miloslav Sanda, Renhuizi Wei, Julius Benicky, Zora Novakova, Cyril Barinka, Radoslav Goldman

Prostate. Author manuscript; available in PMC 2023 Jan 1.

Published in final edited form as: Prostate. 2022 Jan; 82(1): 132–144. Published online 2021 Oct 18. doi: 10.1002/pros.24254 PMCID: PMC9646948

PD-L1 glycosylation and its impact on binding to clinical antibodies

Julius Benicky, Miloslav Sanda, Zuzana Brnakova Kennedy, Oliver C. Grant, Robert J Woods, Alan Zwart, Radoslav Goldman

J Proteome Res. Author manuscript; available in PMC 2022 Jan 1.

Published in final edited form as: J Proteome Res. 2021 Jan 1; 20(1): 485–497. Published online 2020 Oct 19. doi: 10.1021/acs.jproteome.0c00521

PMCID: PMC8158060

Hydrophilic interaction liquid chromatography in the separation of glycopeptides and their isomers

Petr Kozlik, Radoslav Goldman, Miloslav Sanda

Anal Bioanal Chem. Author manuscript; available in PMC 2019 Aug 1.

Published in final edited form as: Anal Bioanal Chem. 2018 Aug; 410(20): 5001–5008. Published online 2018 May 28. doi: 10.1007/s00216-018-1150-3 PMCID: PMC6041177

Changes in the glycosylation of kininogen and the development of a kininogen based algorithm for the early detection of HCC

Mengjun Wang, Miloslav Sanda, Mary Ann Comunale, Harmin Herrera, Charles Swindell, Yuko Kono, Amit G. Singal, Jorge Marrero, Timothy Block, Radoslav Goldman, Anand Mehta

Cancer Epidemiol Biomarkers Prev. Author manuscript; available in PMC 2018 May 1.

Published in final edited form as: Cancer Epidemiol Biomarkers Prev. 2017 May; 26(5): 795–803. Published online 2017 Feb 21. doi: 10.1158/1055-9965.EPI-16-0974

PMCID: PMC5759760

Data Independent Analysis of IgG Glycoforms in Samples of Unfractionated Human Plasma

Miloslav Sanda, Radoslav Goldman

Anal Chem. Author manuscript; available in PMC 2018 Feb 8.

Published in final edited form as: Anal Chem. 2016 Oct 18; 88(20): 10118–10125. Published online 2016 Sep 29. doi: 10.1021/acs.analchem.6b02554 PMCID: PMC5803558

N- and O-Glycosylation of the SARS-CoV-2 Spike Protein

Miloslav Sanda, Lindsay Morrison, Radoslav Goldman

Anal Chem. 2021 Jan 6 : acs.analchem.0c03173. Published online 2021 Jan 6. doi: 10.1021/acs.analchem.0c03173 PMCID: PMC7805595

Low collision energy fragmentation in structure-specific glycoproteomics analysis

Miloslav Sanda, Julius Benicky, Radoslav Goldman

Anal Chem. Author manuscript; available in PMC 2021 Jun 16.

Published in final edited form as: Anal Chem. 2020 Jun 16; 92(12): 8262–8267. Published online 2020 Jun 4. doi: 10.1021/acs.analchem.0c00519 PMCID: PMC8145010

LC-MS³ Quantification of O-Glycopeptides in Human Serum

Miloslav Sanda, Petr Pompach, Julius Benicky, Radoslav Goldman

Electrophoresis. Author manuscript; available in PMC 2014 Aug 1.

Published in final edited form as: Electrophoresis. 2013 Aug; 34(16): 10.1002/elps.201200658. Published online 2013 Jul 24. doi: 10.1002/elps.201200658 PMCID: PMC3808000

Semi-Automated Identification of N-Glycopeptides by Hydrophilic Interaction Chromatography, nano-Reverse-Phase LC-MS/MS, and Glycan Database Search

Petr Pompach, Kevin B. Chandler, Renny Lan, Nathan Edwards, Radoslav Goldman

J Proteome Res. Author manuscript; available in PMC 2013 Mar 2.

Published in final edited form as: J Proteome Res. 2012 Mar 2; 11(3): 1728–1740. Published online 2012 Feb 6. doi: 10.1021/pr201183w  PMCID: PMC3292689 ArticlePubReaderPDF–2.0MCite

Quantification of Fucosylated Hemopexin and Complement Factor H in Plasma of Patients with Liver Disease

Julius Benicky, Miloslav Sanda, Petr Pompach, Jing Wu, Radoslav Goldman

Anal Chem. 2014 Nov 4; 86(21): 10716–10723. Published online 2014 Oct 10. doi: 10.1021/ac502727s

PMCID: PMC4222631 ArticlePubReaderPDF–1.1MCite

Quantitative Liquid Chromatography-Mass Spectrometry-Multiple Reaction Monitoring (LC-MS-MRM) Analysis of Site-specific Glycoforms of Haptoglobin in Liver Disease

Miloslav Sanda, Petr Pompach, Zuzana Brnakova, Jing Wu, Kepher Makambi, Radoslav Goldman

Mol Cell Proteomics. 2013 May; 12(5): 1294–1305. Published online 2013 Feb 6. doi: 10.1074/mcp.M112.023325 PMCID: PMC3650340 ArticlePubReaderPDF–327KCite

Site-Specific Glycan Microheterogeneity of Inter-Alpha-Trypsin Inhibitor Heavy Chain H4

Kevin Brown Chandler, Zuzana Brnakova, Miloslav Sanda, Shuo Wang, Stephanie H. Stalnaker, Robert Bridger, Peng Zhao, Lance Wells, Nathan J. Edwards, Radoslav Goldman

J Proteome Res. 2014 Jul 3; 13(7): 3314–3329. Published online 2014 Jun 2. doi: 10.1021/pr500394z

PMCID: PMC4084840 ArticlePubReaderPDF–2.3MCite

Site-specific Glycoforms of Haptoglobin in Liver Cirrhosis and Hepatocellular Carcinoma

Petr Pompach, Zuzana Brnakova, Miloslav Sanda, Jing Wu, Nathan Edwards, Radoslav Goldman

Mol Cell Proteomics. 2013 May; 12(5): 1281–1293. Published online 2013 Feb 6. doi: 10.1074/mcp.M112.023259 PMCID: PMC3650339 ArticlePubReaderPDF–655KCite

Optimized fragmentation for quantitative analysis of fucosylated N-glycoproteins by LC-MS-MRM

Wei Yuan, Renhuizi Wei, Radoslav Goldman, Miloslav Sanda

Anal Chem. Author manuscript; available in PMC 2020 Jul 16. Published in final edited form as: Anal Chem. 2019 Jul 16; 91(14): 9206–9212. Published online 2019 Jul 3. doi: 10.1021/acs.analchem.9b01983

PMCID: PMC7259371 ArticlePubReaderPDF–1.0MCite

Protein and Site Specificity of Fucosylation in Liver-Secreted Glycoproteins

Petr Pompach, David J. Ashline, Zuzana Brnakova, Julius Benicky, Miloslav Sanda, Radoslav Goldman

J Proteome Res. 2014 Dec 5; 13(12): 5561–5569. Published online 2014 Sep 29. doi: 10.1021/pr5005482

PMCID: PMC4261953 ArticlePubReaderPDF–3.6MCite

Quantitative Analysis of Sex-Hormone-Binding Globulin Glycosylation in Liver Diseases by Liquid Chromatography–Mass Spectrometry Parallel Reaction Monitoring

Wei Yuan, Julius Benicky, Renhuizi Wei, Radoslav Goldman, Miloslav Sanda

J Proteome Res. Author manuscript; available in PMC 2019 Jan 24.

Published in final edited form as: J Proteome Res. 2018 Aug 3; 17(8): 2755–2766. Published online 2018 Jul 16. doi: 10.1021/acs.jproteome.8b00201 PMCID: PMC6344935

Exploring Site-Specific N-Glycosylation Microheterogeneity of Haptoglobin using Glycopeptide CID Tandem Mass Spectra and Glycan Database Search

Kevin Brown Chandler, Petr Pompach, Radoslav Goldman, Nathan Edwards

J Proteome Res. Author manuscript; available in PMC 2014 Aug 2.

Published in final edited form as: J Proteome Res. 2013 Aug 2; 12(8): 3652–3666. Published online 2013 Jul 22. doi: 10.1021/pr400196s PMCID: PMC3777712

Study of structure-dependent chromatographic behavior of glycopeptides using reversed phase nanoLC

Petr Kozlik, Radoslav Goldman, Miloslav Sanda

Electrophoresis. Author manuscript; available in PMC 2018 Sep 1.

Published in final edited form as: Electrophoresis. 2017 Sep; 38(17): 2193–2199. Published online 2017 May 17. doi: 10.1002/elps.201600547 PMCID: PMC5581245

The conserved oligomeric Golgi complex is required for fucosylation of N-glycans in Caenorhabditis elegans

Weston B Struwe, Vernon N Reinhold

Glycobiology. 2012 Jun; 22(6): 863–875. Published online 2012 Feb 28. doi: 10.1093/glycob/cws053

PMCID: PMC3336869

A Quantitative Microtiter Assay for Sialylated Glycoform Analyses Using Lectin Complexes

Karunya Srinivasan, Sucharita Roy, Nathaniel Washburn, Sandra F. Sipsey, Robin Meccariello, James W. Meador, III, Leona E. Ling, Anthony M. Manning, Ganesh V. Kaundinya

J Biomol Screen. 2015 Jul; 20(6): 768–778. doi: 10.1177/1087057115577597

PMCID: PMC4512520

RAPID RELEASE OF N-LINKED GLYCANS FROM GLYCOPROTEINS BY PRESSURE CYCLING TECHNOLOGY

Zoltan Szabo, András Guttman, Barry L. Karger

Anal Chem. Author manuscript; available in PMC 2011 Mar 15.

Published in final edited form as: Anal Chem. 2010 Mar 15; 82(6): 2588–2593. doi: 10.1021/ac100098e

PMCID: PMC2847834